Author

Nipam Patel
http://patellab.org     Email

Published on SDB CoRe: Oct 3 2012

Organisms: Model Organisms; Invertebrates
Tools & Techniques: Visualizing Genes/ Proteins; Microscopy
Embryonic Patterning: Segmentation; Compartments/Boundaries
Organism: Drosophila
Stage of Development: Embryo

Object Description

Drosophila embryo (stage 11) immunostained for the segment polarity gene products Engrailed (En, orange) and Cubitis interruptus (Ci, green), and the homeotic gene product Ultrabithorax (Ubx, pink).  All nuclei stained with DAPI (blue).  Lateral view, anterior to left, ventral down.  Ci is expressed in the anterior compartment of each segment, while En is expressed in the posterior compartment.  These and other segment polarity genes maintain boundaries between segmental units, and refine patterns within each segment. Ubx is expressed in a portion of parasegment 5, most predominately in parasegment 6 (PS6), and throughout parasegments 7-12.  Homeotic genes such as Ubx establish regional identity and help give each segment its unique identity.

References

Gilbert, S.F. Developmental Biology. 6th edition. Sunderland, MA, Sinauer Associates, 2000. The Origins of Anterior-Posterior Polarity. http://www.ncbi.nlm.nih.gov/books/NBK10039/.

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