Author

MBL Embryology
http://mblembryology.org     Email

Additional Author(s): Samantha Jones

Published on SDB CoRe: Sep 28 2012

Organisms: Model Organisms; Vertebrates
Tools & Techniques: Microscopy
Mesoderm-derived: Somites; Limb Formation; Skeleton
Ectoderm-derived: Neural Crest
Organism: Mouse
Stage of Development: Larva/Juvenile

Object Description

Skeleton preparation of a newborn mouse.  Alizarin red was used to detect bone, and alcian blue for cartilage.  To ensure that all parts of the preparation were in focus, multiple images were collected at different focal planes and an extended focus image was generated using Helicon Focus software.  To maintain as much detail as possible in the lighter and darker regions, mutiple exposures were also collected and processed using High Dynamic Range imaging.

References

Gilbert, S.F. Developmental Biology. 6th edition. Sunderland, MA, Sinauer Associates, 2000. Osteogenesis: The Development of Bones. http://www.ncbi.nlm.nih.gov/books/NBK10056/.

Gilbert, S.F. Developmental Biology. 6th edition. Sunderland, MA, Sinauer Associates, 2000. The Neural Crest. http://www.ncbi.nlm.nih.gov/books/NBK10065/.

Gilbert, S.F. Developmental Biology. 6th edition. Sunderland, MA, Sinauer Associates, 2000. Formation of the Limb Bud. http://www.ncbi.nlm.nih.gov/books/NBK10003/.

Comment on this Object

You must be logged in to CoRe to comment. Please login or create an account.